immoil f30cc low auto fluorescence type f immersion oil  (Olympus)

Journal: Nature protocols

Article Title: High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation

doi: 10.1038/s41596-020-00440-x

Figure Lengend Snippet: Troubleshooting table

Article Snippet: Olympus IX81 equipped with a 1.4-NA ×100 oil-immersion apochromatic objective (UPLSAPO 100XO, Olympus) or any suitable inverted fluorescence microscope Low autofluorescence immersion oil (Olympus, cat. no. IMMOIL-F30CC)

Techniques: Fluorescence, Microscopy, Imaging, Concentration Assay, Labeling, Refractive Index, Diffusion-based Assay

Journal: STAR Protocols

Article Title: Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts

doi: 10.1016/j.xpro.2021.101119

Figure Lengend Snippet: Optical setup of fluorescence microscope (A) Excitation spectra of fluorophores with complementary excitation filters. (B) Transmission spectra of dichroic filter. (C) Emission spectra of fluorophores with complementary emission filter.

Article Snippet: Low Fluorescence Immersion Oil (Type FF) , Cargille , Cat#: 16212.

Techniques: Fluorescence, Microscopy, Transmission Assay

Journal: STAR Protocols

Article Title: Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts

doi: 10.1016/j.xpro.2021.101119

Figure Lengend Snippet: On Stage calibration of Grx1roGFP2 Individual wavelength responses of a single HepG2 cell expressing ER-M Grx1roGFP2 & OMM-Grx1roGFP2 targeted to the ER-mitochondrial interface. Grx1 is fully reduced through addition of 1mM DTT. A second DTT addition is used to ensure complete reduction of the probe as shown by increase of the F480 emission signal and decrease of the F402 nm signal shown as fluorescence arbitrary units. The DTT containing imaging solution is replaced by multiple washes and addition of 200μM H 2 O 2 . A second pulse of H 2 O 2 is added to ensure full oxidation as shown by a decrease of the F480 nm emission and increase of the F402 nm emission.

Article Snippet: Low Fluorescence Immersion Oil (Type FF) , Cargille , Cat#: 16212.

Techniques: Expressing, Fluorescence, Imaging

Journal: STAR Protocols

Article Title: Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts

doi: 10.1016/j.xpro.2021.101119

Figure Lengend Snippet: Examples of mitochondrial flicker analysis (A) two flickers of the same organelle, A and B identified by difference image analysis. Each have stable pre-flicker fluorescence values (F0A and F0B), maximum depolarization (FminA and FminB). Half-Max depolarization is calculated and marked for each flicker (Half-MaxA and Half-MaxB). Initiation for both flickers is derived from the time of Half-Max depolarization. (B) Fluorescence values and time points given for flickers A and B in panel A.

Article Snippet: Low Fluorescence Immersion Oil (Type FF) , Cargille , Cat#: 16212.

Techniques: Fluorescence, Derivative Assay

Journal: STAR Protocols

Article Title: Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts

doi: 10.1016/j.xpro.2021.101119

Figure Lengend Snippet: Mitochondrial membrane flickers and oxidative bursts evoked in HepG2 cells after stimulation with Staurosporine (A) Example images from Difference Image Analysis. showing individual flickers and regions of interest (ROIs) selected around them. Area based on 0.4μm 2 pixel size. (B) Graph showing area of flickers Vs. time following stimulation (Staurosporine, 2μM at t= 5mins) (C) Sample traces of repeated oxidative bursts derived from flicker ROI masking of Grx1roGFP2 fluorescence emission.

Article Snippet: Low Fluorescence Immersion Oil (Type FF) , Cargille , Cat#: 16212.

Techniques: Membrane, Derivative Assay, Fluorescence

Journal: STAR Protocols

Article Title: Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts

doi: 10.1016/j.xpro.2021.101119

Figure Lengend Snippet: Potential pitfalls of imaging mitochondrial membrane potential with TMRM (A) Cells treated with a depolarization cocktail (FCCP: 5 μM & Oligomycin: 2.5 μg/μL) show an immediate transition from a mitochondrial fluorescence distribution (t = 0 min) to a less-specific whole-cell fluorescence (t = 1 min). Following rapid loss of the mitochondrial distribution, TMRM fluorescence persists for many minutes (t = 5 min). Scale bar: 10 μm. (B) Line graph depiction of (A) in which FCCP & Oligomycin (addition indicated by arrow at 0 min) cause a rapid redistribution of TMRM fluorescence from the mitochondrial matrix (Red) to the cytosol (blue) where it is slowly lost from the whole cell region (Black). Detail of the redistribution is shown in expanded timescale (total: 90 s. Inset). The loss of TMRM fluorescence from the whole cell region may have a slow kinetic generating an artificially high F min (Dashed line). Replenishment of the imaging buffer (arrow at 6:30 s) speeds TMRM loss to generate a more accurate F min (Dotted line). (C) Section of a HepG2 cell undergoing mitochondrial flickers stimulated by staurosporine. Transient loss of ΔѰm causes a rapid loss of TMRM fluorescence (M1, t = 19, pseudo color difference: Red) in the active mitochondrion. TMRM leaving the flickering mitochondrion is visible as a cloud of increased fluorescence in the surrounding cytosol (t = 19 pseudo color: Blue). Cytosolic fluorescence of released TMRM decreases (t = 21 s pseudo color: Red) as it is taken up by neighboring polarized mitochondria (M2, t = 21 s pseudo color: Blue), which declines upon the repolarization of the flickering mitochondrion (M1, Blue, M2, Red). Scale Bar 5μm. (D) Line graph of 3 regions of interest (Flickering mitochondrion: M1, stable mitochondrion: M2 & extramitochondrial region of cytosol: Cyto). Demonstrating loss of TMRM from M1 to the cytosol and subsequent uptake to the polarized M2. Restoration to the starting distribution begins after repolarization of M1 (t = 20 s).

Article Snippet: Low Fluorescence Immersion Oil (Type FF) , Cargille , Cat#: 16212.

Techniques: Imaging, Membrane, Fluorescence

Journal: STAR Protocols

Article Title: Fluorescence imaging detection of nanodomain redox signaling events at organellar contacts

doi: 10.1016/j.xpro.2021.101119

Figure Lengend Snippet:

Article Snippet: Low Fluorescence Immersion Oil (Type FF) , Cargille , Cat#: 16212.

Techniques: Recombinant, Saline, Software, Inverted Microscopy, Microscopy, Cell Culture, Transferring, Cell Counting, Fluorescence